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rEcoli XpS

Bacterial Strain

#192872

Purpose

MG1655-C321 -- All 321 UAG codons changed to UAA

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only
HL 932

Bacterial Strain

#52940

Purpose

MG1655 + ΔlacI

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Bacterial Resistance

None

Species

Escherichia coli

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
JM110

Bacterial Strain

#49763

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Bacterial Resistance

Streptomycin

Available since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only
B-95.ΔAΔfabR

Bacterial Strain

#197934

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only
C321.ΔA.exp

Bacterial Strain

#49018

Purpose

This is C321.DA with mutS reverted and the lambda prophage removed; it exhibits lesser spontaneous mutagenesis and can be grown at 37 C

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
C321.ΔA

Bacterial Strain

#48998

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted)

Depositor

George Church

Article

Lajoie et al Science. 2013 Oct 18;342(6156):357-60. d

Bacterial Resistance

Ampicillin

Species

Escherichia coli

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
C-5545 ∆cosδε

Bacterial Strain

#209018

Purpose

Deficient in the P2 bacteriophage packaging cos site and encoding rhamnose-inducible P4 delta (δ) and epsilon (ε) genes

Depositor

Alfonso Jaramillo

Article

Tridgett et al ACS Synth Biol. 2021 Jan 15;10(1):107-11

Bacterial Resistance

Hygromycin

Species

Escherichia coli

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only
S1030

Bacterial Strain

#105063

Purpose

host strain for phage assisted continuous evolution

Depositor

David Liu

Article

Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

Bacterial Resistance

Streptomycin and tetracycline

Available since

March 21, 2018

Availability

Academic Institutions and Nonprofits only
Jun lab tunable CRISPRi strain

Bacterial Strain

#86400

Purpose

Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

Depositor

Suckjoon Jun

Article

Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

Bacterial Resistance

Spectinomycin and hygromycin

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
LC-E02

Bacterial Strain

#78551

Purpose

E. coli strain K-12 MG1655, pTet--dCas9 cassette integrated at 186 primary attB site.

Depositor

David Bikard

Article

Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

Bacterial Resistance

None

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only
S2060

Bacterial Strain

#105064

Purpose

host strain for phage assisted continuous evolution

Depositor

David Liu

Article

Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

Bacterial Resistance

Streptomycin and tetracycline

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only
JM107

Bacterial Strain

#49759

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
BL21(DE3)-R3-pRARE2

Bacterial Strain

#26242

Purpose

Phage-resistant derivative of BL21(DE3), which has been transformed with plasmid pRARE2, which carries seven rare-codon tRNA genes.

Depositor

Opher Gileadi

Article

Savitsky et al J Struct Biol. 2010 Jun 10. ():.

Bacterial Resistance

Chloramphenicol

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only
RHO3

Bacterial Strain

#124700

Purpose

E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

Depositor

Herbert Schweizer

Article

Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

Bacterial Resistance

Dap (400 µg/ml)

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only
C321.∆A.opt

Bacterial Strain

#87359

Purpose

This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

Depositor

George Church

Article

Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

Bacterial Resistance

Gentamicin

Species

Synthetic

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only
JM101

Bacterial Strain

#50349

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Messing et al Recombinant DNA Technical Bulletin, 1979

Bacterial Resistance

None

Species

Escherichia coli

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
JM109

Bacterial Strain

#49761

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Yanisch-Perron et al Gene. 1985;33(1):103-19.

Bacterial Resistance

None

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
E. coli K-12 MG1655 RARE

Bacterial Strain

#61440

Purpose

This E. coli chassis displays reduced endogenous reduction of aromatic and aliphatic aldehydes

Depositor

Kristala Prather

Article

Kunjapur et al J Am Chem Soc. 2014 Aug 20;136(33):11644

Bacterial Resistance

None

Available since

Feb. 1, 2015

Availability

Academic Institutions and Nonprofits only
JM83

Bacterial Strain

#50348

Purpose

E.coli K-12 derivative

Depositor

Joachim Messing

Article

Messing et al Recombinant DNA Technical Bulletin, 1979

Bacterial Resistance

None

Species

Escherichia coli

Available since

Oct. 14, 2014

Availability

Academic Institutions and Nonprofits only
pRK24

Bacterial Strain

#51950

Purpose

broad-host range natural conjugative plasmid

Depositor

Farren Isaacs

Article

Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

Bacterial Resistance

Tetracycline

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 20 of 408 results

Results per page:

Sort results by:

rEcoli XpS

HL 932

JM110

B-95.ΔAΔfabR

C321.ΔA.exp

C321.ΔA

C-5545 ∆cosδε

S1030

Jun lab tunable CRISPRi strain

LC-E02

S2060

JM107

BL21(DE3)-R3-pRARE2

RHO3

C321.∆A.opt

JM101

JM109

E. coli K-12 MG1655 RARE

JM83

pRK24

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